New SIMEX-based research paper just published in Structural Dynamics
A new peer-reviewed open access paper has been released in the Structural Dynamics journal, as a result of the work conducted in PaNOSC to setup and develop tools for simulation of experiments at PaN facilities.
ABSTRACT
The unprecedented intensity of x-ray free-electron laser sources has enabled single-particle x-ray diffraction imaging (SPI) of various biological specimens in both two-dimensional projection and three dimensions (3D). The potential of studying protein dynamics in their native conditions, without crystallization or chemical staining, has encouraged researchers to aim for increasingly higher resolutions with this technique. The currently achievable resolution of SPI is limited to the sub-10 nanometer range, mainly due to background effects, such as instrumental noise and parasitic scattering from the carrier gas used for sample delivery. Recent theoretical studies have quantified the effects of x-ray pulse parameters, as well as the required number of diffraction patterns to achieve a certain resolution, in a 3D reconstruction, although the effects of detector noise and the random particle orientation in each diffraction snapshot were not taken into account. In this work, we show these shortcomings and address limitations on achievable image resolution imposed by the adaptive gain integrating pixel detector noise.
Article reference:
Juncheng E, Y. Kim, J. Bielecki, M. Sikorski, R. de Wijn, C. Fortmann-Grote, J. Sztuk-Dambietz, J. C. P. Koliyadu, R. Letrun, H. J. Kirkwood, T. Sato, R. Bean, A. P. Mancuso, and C. Kim, Expected resolution limits of x-ray free-electron laser single-particle imaging for realistic source and detector properties, Structural Dynamics 9, 064101 (2022); https://doi.org/10.1063/4.0000169
